The high astringency intensity in Gujing tea could be due to the high accumulation of proanthocyanidins and galloylquinic acid. The results of the present study may improve our understanding of the metabolic characteristics of each evolutionary group of species or varieties in the section Thea of the genus Camellia.The possibility of combining the health benefits of kafirin and polyphenols and improving the bioavailability of resveratrol using hollow kafirin nanoparticles via gallic acid crosslinking was investigated. The size, morphology, charge state, loading efficiency, physicochemical stability, and redispersity after lyophilization of hollow resveratrol-loaded kafirin nanoparticles formed via gallic acid crosslinking were characterized and compared with their solid counterparts and those without crosslinking. The nanoparticles formed were anionic spheres with an average diameter of less then 100 nm when loading amounts of resveratrol were less than 20%. The hollow nanoparticles were homogenous and still achieved stable colloidal dispersion after lyophilization. The hollow nanoparticles crosslinked with gallic acid displayed stability against pancreatin and delayed release in stimulated digestion. The results suggested that hollow kafirin nanoparticles could be a favorable colloidal delivery system for incorporating resveratrol.The use of starter cultures helps to improve the quality and safety of traditional meat products. In this study, the effects of Lactobacillus plantarum R2 and Staphylococcus xylosus A2 inoculation on microbial community, lipolysis, proteolysis, and volatile compounds in Chinese dry fermented sausages were investigated. Culture-dependent and culture-independent high-throughput sequencing) methods were employed to evaluate the bacterial communities. Results showed that the total contents of free fatty acids (FFAs) and free amino acids (FAAs) were increased by inoculating the starter cultures, especially a mixed culture. A significant decrease in pH and water activity was observed in the inoculated sausages (p less then 0.05). Moreover, the inoculation treatment enhanced the competitiveness of dominant bacteria and inhibited the growth of unwanted bacteria. This was beneficial to promote the release of FFAs and FAAs and to prevent the formation of off-flavours and rancidity. Thus, the flavour development in the inoculated dry fermented sausages was attributed to the improvements in microbiological quality.Background Organ shortages limit the progress of organ transplantation. The family attitudes of patients with end-stage renal disease (ESRD) play an important role in advocating organ transplantation and donation. The purpose of this study was to analyze the family attitudes of patients with ESRD toward living kidney donation in China. Ethical approval was obtained from the ethics committee of Linyi People's Hospital. This study was performed in compliance with the Declaration of Helsinki. Materials and methods This research was performed at 5 third-level hospitals with hemodialysis and nephrology departments, and a small section comes from urology departments. The participants were surveyed from January to November 2018. Attitudes were evaluated using a validated questionnaire concerning the psychosocial aspects of organ donation. The self-administered questionnaire was completed anonymously. Statistical analyses employed t tests and the χ2 test. Results Regarding living kidney donation, 69.1% (n = 428) of patient families favored it; however, only 30.9% (n = 192) did not support it. A favorable attitude toward living donation was mainly associated with the following variables 1. the recipient is not more than 50 years old; 2. the recipient is a member of the immediate family; 3. the living donation is from the recipient's family member; 4. the family has previous personal experience with organ transplantation and donation; and 5. the family has a concern about the possibility of needing a transplant within the family unit (P less then .05). Conclusions Economic burden and mental stress from long-term dialysis influenced the attitudes and behavioral intentions of the families of patients with ESRD on advocating organ donation. Repeated education and constant advocacy are advised to increase the participation of families of patients with ESRD in organ donation. The results showed favorable attitudes toward living kidney donation among the families of patients with ESRD.Introduction The extracellular matrix (ECM) consists, among others, of polysaccharides, glycosaminoglycans, and proteins. It is being increasingly used in tissue bioengineering. Obtaining ECM of the highest quality through decellularization is a big challenge because of some differences in organ structure. To deprive organs of the cellular part, chemical, enzymatic, or mechanical methods are used. After decellularization, we get a scaffold made of a variety of proteins, and it is the role of these proteins that can significantly affect the maintenance of the spatial structure and be a suitable environment for cells to rebuild a specific organ. Aim Estimation of the detergent (Triton X-100) flow parameters and anthropometric donors' decellularization process accuracy on the final ECM composition. Materials Five human pancreata, rejected from transplantation, were used for decellularization. All organs were harvested from brain-dead donors age 13 to 60 years. Methods Decellularization was carried out using the flow method with Triton X-100 as an active agent. Necrostatin-1 RIP kinase inhibitor The experiment compared 5 different flow values. After decellularization, an assessment of the final DNA concentration and the protein composition was performed. Results were compared to anthropometric data of donors. In addition, a microscopic analysis was also carried out. Results The best results were obtained using a flow of 120 mL/minute. A higher detergent flow was associated with a lower concentration of residual DNA in scaffold. Analysis of the protein profile with anthropometric data has shown that LAM A2 was increasing with age and LAMA5 was decreasing. Being overweight was associated with a higher proportion of COL1 and 4 and a smaller proportion of COL6.Necrostatin-1 RIP kinase inhibitor